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Tissue-Specific Gamma-Flicker Light Noninvasively Ameliorates Retinal Aging.

Wang Sheng1,2, Da Lv1,2, Ze-Kai Cui2

  • 1Aier School of Ophthalmology, Central South University, Changsha, Hunan, China.

Cellular and Molecular Neurobiology
|October 26, 2021
PubMed
Summary

Gamma-flicker light (γFL) treatment reduced retinal aging markers in mice. This noninvasive therapy improved retinal structure and function, offering a potential strategy for age-related retinal diseases.

Keywords:
AgingMitochondrial disordersMolecular chaperonesPhototherapyRetina

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Area of Science:

  • Ophthalmology and Neuroscience
  • Gerontology and Regenerative Medicine

Background:

  • Aging is a significant risk factor for various retinal degenerative diseases.
  • Gamma-flicker light (γFL) has shown promise in mitigating Alzheimer's disease pathology in mouse models.
  • The direct impact of γFL on retinal aging processes remained unexplored.

Purpose of the Study:

  • To investigate the direct effects of gamma-flicker light (γFL) on age-related changes in the mouse retina.
  • To explore the potential of γFL as a therapeutic strategy for retinal aging.

Main Methods:

  • Assessment of senescence-associated beta-galactosidase (β-gal) and autofluorescence in aged mice treated with γFL.
  • Immunofluorescence and Western blotting to evaluate aging-related protein expression and cellular changes.
  • mRNA sequencing to identify underlying molecular mechanisms, including mitochondrial function and protein folding.
  • Treatment of aged Apoe-/- mice with γFL to assess structural improvements in the retinal pigment epithelium and Bruch's membrane.

Main Results:

  • γFL treatment reduced β-gal-positive cells and lipofuscin accumulation in the aged mouse retina.
  • γFL ameliorated declines in microtubule-associated protein 1 light chain 3 beta expression and complement C3 activity.
  • γFL rebalanced antioxidant and pro-oxidant factors and modulated activating transcription factor 4 (ATF4) expression.
  • mRNA sequencing revealed impacts on mitochondrial function and protein folding pathways.
  • γFL treatment improved retinal pigment epithelium basal infolding and Bruch's membrane structures in aged Apoe-/- mice.

Conclusions:

  • Gamma-flicker light (γFL) effectively mitigates multiple pathological characteristics associated with retinal aging in mice.
  • γFL demonstrates potential as a noninvasive, convenient, and tissue-specific therapeutic approach for age-related retinal degeneration.