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Recent progress on n-butanol production by lactic acid bacteria.
Qi Li1,2, Jieze Zhang3, Junjie Yang2
1College of Life Sciences, Sichuan Normal University, Chengdu, 610101, China.
World Journal of Microbiology & Biotechnology
|October 26, 2021
Summary
Lactic acid bacteria (LAB) show high resistance to n-butanol, making them ideal for improving its microbial fermentation. This review explores LAB tolerance, genome editing, and modification strategies for enhanced n-butanol production.
Area of Science:
- Biotechnology
- Microbial Fermentation
- Metabolic Engineering
Background:
- n-Butanol is a vital chemical intermediate produced via microbial fermentation.
- Cellular toxicity of n-butanol significantly hinders its efficient production.
- Lactic acid bacteria (LAB) exhibit superior n-butanol tolerance, positioning them as key candidates for production enhancement.
Purpose of the Study:
- To review the n-butanol tolerance of LAB, including levels and mechanisms.
- To examine genome editing tools applicable to n-butanol-resistant LAB.
- To summarize LAB modification methods and achieved production levels for n-butanol.
Main Methods:
- Literature review focusing on n-butanol production by LAB.
- Analysis of studies on LAB tolerance mechanisms to n-butanol.
- Compilation of data on genome editing and metabolic modification techniques in LAB.
Main Results:
- LAB demonstrate significant resistance to n-butanol, with varying tolerance levels reported.
- Established genome editing tools are available for modifying n-butanol-resistant LAB.
- Various modification strategies have led to improved n-butanol production yields in LAB.
Conclusions:
- LAB represent a promising platform for sustainable n-butanol production due to their inherent tolerance.
- Further research leveraging genome editing and metabolic engineering in LAB can optimize n-butanol biosynthesis.
- This review provides a foundational understanding for advancing n-butanol production using LAB.
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