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Updated: Oct 15, 2025
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
Published on: August 9, 2019
Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
Aina Segués1, Sander M J van Duijnhoven2, Marc Parade2
1Aduro Biotech Europe, Oss, the Netherlands; Faculty of Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, the Netherlands; Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, United Kingdom.
Abstract:
Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
Insights
Researchers developed new antibodies targeting mouse Tumor Necrosis Factor Receptor 2 (TNFR2). These tools help study TNFR2
Area of Science:
- Immunology
- Molecular Biology
- Reagent Development
Background:
- Tumor necrosis factor receptor 2 (TNFR2) is implicated in autoimmune diseases and cancer.
- TNFR2's precise role in regulating immune cells remains unclear.
- Well-characterized reagents are needed to selectively modulate TNFR2 function.
Purpose of the Study:
- To generate and characterize novel antibodies targeting mouse TNFR2.
- To enable the study of TNFR2-dependent biological processes in preclinical models.
Main Methods:
- Generation, production, purification, and characterization of anti-mouse TNFR2 antibodies.
- Assessment of antibody binding affinity, TNFα blocking potency, and epitope mapping.
- Evaluation of antibody effects on CD8+ T-cell activation and Treg binding ex vivo.
Main Results:
- A panel of novel antibodies targeting mouse TNFR2 was successfully generated.
- Antibodies exhibited functional differences in binding affinity and TNFα blocking capacity.
- Antibodies targeted distinct TNFR2 domains, influencing CD8+ T-cell activation and costimulation.
- Antibodies bound to splenic Tregs and activated CD8+ T-cells ex vivo.
Conclusions:
- Novel anti-mTNFR2 antibodies provide valuable tools for investigating TNFR2 biology.
- These antibodies can modulate TNFR2 function, aiding research in autoimmunity and cancer models.
- The characterized antibodies facilitate the study of TNFR2-mediated immune responses.
