Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hamster sperm cross react with antiactin.

P Talbot, M G Kleve

    The Journal of Experimental Zoology
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Hamster sperm contain actin, a protein also found in muscle. This actin is located in various parts of the sperm, suggesting a role in fertilization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of a Resonant Microwave Sensor for Sediment Density Characterization.

    Sensors (Basel, Switzerland)·2020
    Same author

    Ultrastructure and role of the lobster vas deferens in spermatophore formation: The proximal segment.

    Journal of morphology·2018
    Same author

    A comprehensive analysis of the evaporation of a liquid spherical drop.

    Journal of colloid and interface science·2014
    Same author

    Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids.

    Toxicology in vitro : an international journal published in association with BIBRA·2014
    Same author

    What knowledge and attitudes do paid carers of people with a learning disability have about cancer?

    European journal of cancer care·2013
    Same author

    A method for rapid dose-response screening of environmental chemicals using human embryonic stem cells.

    Journal of pharmacological and toxicological methods·2012

    Area of Science:

    • Reproductive biology
    • Cell biology
    • Biochemistry

    Background:

    • Actin is a key protein in muscle contraction and cell structure.
    • The presence and function of actin in sperm are not fully understood.

    Purpose of the Study:

    • To investigate the presence of actin in hamster sperm.
    • To determine the localization of actin within hamster sperm.

    Main Methods:

    • Polyacrylamide gel electrophoresis to compare sperm extract polypeptide with muscle actin.
    • Double diffusion precipitation assays using an antibody to muscle actin (antiactin).
    • Indirect immunofluorescent microscopy to visualize antiactin binding sites in sperm.

    Main Results:

    • A polypeptide in hamster sperm extracts comigrated with muscle actin on gels.

    Related Experiment Videos

  • Sperm extracts showed a reaction of identity with muscle actin using antiactin.
  • Antiactin localized to the acrosomal region, neck, and tail of hamster sperm.

    • Conclusions:

    • Hamster sperm contain actin, a protein structurally similar to muscle actin.
    • The localization suggests a potential role for sperm actin in fertilization processes.