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Cellular behaviour in epithelial root sheath following vinblastine administration in mouse
Journal De Biologie Buccale
|March 1, 1987
Summary
Vinblastine sulfate (VLB) affects epithelial root sheath (ERS) cells, promoting basal lamina formation and suggesting a role in root morphogenesis. VLB also questions the ERS
Area of Science:
- Cell Biology
- Developmental Biology
- Histology
Background:
- The epithelial root sheath (ERS) is crucial for root development.
- Understanding ERS cell behavior is key to comprehending root morphogenesis.
Purpose of the Study:
- To investigate the effects of vinblastine sulfate (VLB) on epithelial root sheath (ERS) cells.
- To elucidate the role of ERS in basal lamina formation and root morphogenesis.
Main Methods:
- Administration of vinblastine sulfate (VLB) to induce cellular changes.
- Microscopic analysis of epithelial root sheath (ERS) cells and surrounding tissues.
- Assessment of cell contacts, vesicle formation, and mitotic activity.
Main Results:
- VLB induced phagosome and lysosome accumulation, and exocytosis vesicles in ERS cells.
- Increased cell contacts between pulp mesenchyme and ERS were observed.
- Mitotic figures arrested at prometaphase, with a higher index at the sheath tip.
Conclusions:
- ERS actively participates in basal lamina formation during root morphogenesis.
- Heterogeneous cell relations are common in root development.
- The role of ERS in cementogenesis is questioned, while its mitotic potential may relate to odontoblast differentiation.