Actinomadura soli sp. nov., isolated from the top soil layer on basaltic material in Turkey

An actinobacterium, designated 14C53^T, was isolated from a soil sample on basaltic material from Samsun, Turkey. The growth ranges for NaCl concentration and pH of strain 14C53^T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53^T was most closely related to Actinomadura geliboluensis A8036^T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310A^T. The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383^T. However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53^T with Actinomadura geliboluensis A8036^T and Actinomadura pelletieri DSM 43383^T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura. The genome size of strain 14C53^T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%. The major cellular fatty acids of strain 14C53^T were C_16 : 0 and iso-C_16 : 0. Strain 14C53^T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan. The predominant menaquinones were MK-9(H₈) and MK-9(H₆). Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp. nov. is proposed, with 14C53^T (=DSM 104447^T=KCTC 39878^T) as the type strain.