Reduction of Recombinant Adeno-Associated Virus Vector Adsorption on Solid Surfaces by Polyionic Hydrophilic Complex...

Area of Science:

• Gene Therapy
• Biotechnology
• Molecular Biology

Background:

• Recombinant adeno-associated virus (rAAV) vectors are vital for gene therapy.
• Non-specific adsorption of rAAV vectors to surfaces causes significant particle loss (up to 90%) and quantification variability.
• This loss impacts production, storage, administration, and accurate quantification processes.

Purpose of the Study:

• To develop and evaluate a method for reducing rAAV vector adsorption on material surfaces.
• To improve the recovery and quantification accuracy of rAAV vectors.

Main Methods:

• Application of a polyionic hydrophilic complex polymer coating on surfaces of lab tools (pipette tips, cryotube vials, qPCR plates).
• Quantitative polymerase chain reaction (qPCR) analysis to measure vector recovery.
• In vitro transduction efficiency assays to assess functional recovery.

Main Results:

• The polymer coating significantly decreased rAAV vector adsorption.
• Up to 95% of vector particle loss was prevented by using coated tools.
• Coated tools led to higher recovery of functional vector particles, evidenced by improved in vitro transduction efficiency.

Conclusions:

• The tested polyionic hydrophilic complex polymer coating is effective in minimizing rAAV vector loss on surfaces.
• This coating offers a practical solution for improving vector recovery and quantification in gene therapy applications.
• The technology has potential for widespread use in gene therapy material processing.