Analysis of PDA Dose Curves for the Extraction of Antimicrobial Peptide Properties

A mechanochromic polymer, polydiacetylene, changes color upon ligand binding, being a popular material in biosensing. However, whether it can also detect ligand functions in addition to binding is left understudied. In this work, we report that the polydiacetylene can be used to determine the net charges and the mode of actions (carpet model, toroidal pore model, etc.) of antimicrobial peptides and detergents via EC₅₀ and Hill coefficients from the colorimetric response-dose curves. This opens a potential for high-throughput peptide screening by functions, which is difficult with the conventional methods.