Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nesting: hierarchies of allosteric interactions.

C H Robert, H Decker, B Richey

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1987
    PubMed
    Summary

    This study introduces a generalized allosteric model accounting for hierarchical conformational equilibria in macromolecules. This model explains the binding properties of hemocyanin proteins with multiple binding sites.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    2013 SYR Accepted Poster Abstracts.

    International journal of yoga therapy·2013
    Same author

    Nasopharyngeal rhabdomyosarcoma.

    Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India·2012
    Same author

    Synthetic approaches to bicyclic diazenium salts.

    The Journal of organic chemistry·2010
    Same author

    NEUROID TRANSMISSION IN CILIATED EPITHELIUM.

    The Journal of general physiology·2009
    Same author

    Pore formation kinetics in membranes, determined from the release of marker molecules out of liposomes or cells.

    Biophysical journal·2009
    Same author

    Senile hair graying: H2O2-mediated oxidative stress affects human hair color by blunting methionine sulfoxide repair.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2009

    Area of Science:

    • Biophysics
    • Structural Biology
    • Biochemistry

    Background:

    • Allosteric models describe the regulation of protein function through conformational changes.
    • Large macromolecular systems often exhibit complex hierarchical structural organization.
    • Understanding these structures is key to interpreting their functional binding properties.

    Purpose of the Study:

    • To present a generalized allosteric model incorporating hierarchical conformational equilibria.
    • To apply this model to understand the structural and functional aspects of hemocyanin.
    • To interpret the binding properties of hemocyanin systems with numerous binding sites.

    Main Methods:

    • Development of a generalized allosteric model.
    • Incorporation of a hierarchy of conformational equilibria.

    Related Experiment Videos

  • Application of 'nested' models to analyze hemocyanin systems.

    • Main Results:

    • The generalized model successfully integrates hierarchical conformational equilibria.
    • Functional binding properties are shown to reflect equilibria at each structural level.
    • The model effectively interprets structural features and functional aspects of hemocyanin.

    Conclusions:

    • The generalized allosteric model provides a robust framework for complex macromolecular systems.
    • Hierarchical conformational equilibria are crucial for understanding protein function.
    • This approach offers insights into the multi-site binding mechanisms of hemocyanin.