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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study

Akankwasa Gilbert1, An Changjuan1, Cheng Guixue1

  • 1Department of Clinical Laboratory, Shengjing Hospital of China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, Liaoning Province 110004, China.

Disease Markers
|October 28, 2021
PubMed
Abstract

Insights

Urinary matrix metalloproteinase-9 (MMP-9) and nephrin are elevated in idiopathic membranous nephropathy (IMN). MMP-9 shows greater potential than nephrin for monitoring IMN severity and kidney function.

Area of Science:

  • Nephrology
  • Biomarker Discovery
  • Glomerular Diseases

Background:

  • Idiopathic membranous nephropathy (IMN) prognosis relies on proteinuria, which lags behind kidney injury.
  • Urinary matrix metalloproteinase-9 (MMP-9) and nephrin may indicate disease severity in IMN-like conditions.
  • The role of these biomarkers in IMN, a primary cause of adult nephrotic syndrome, is largely unknown.

Purpose of the Study:

  • To investigate the potential of urinary MMP-9 and nephrin as biomarkers in IMN.
  • To assess the relationship between these biomarkers and key renal function parameters.

Main Methods:

  • Quantified urinary MMP-9 and nephrin in 107 IMN patients and 70 healthy controls using ELISA.
  • Compared biomarker levels between groups and correlated them with proteinuria and estimated glomerular filtration rate (eGFR).

Main Results:

  • Urinary MMP-9 and nephrin levels were significantly higher in IMN patients versus controls.
  • MMP-9, but not nephrin, correlated significantly with proteinuria and was higher in nephrotic-range proteinuria.
  • Both biomarkers correlated with eGFR, with MMP-9 significantly elevated in patients with eGFR < 90 ml/min/1.73 m².

Conclusions:

  • Urinary MMP-9 demonstrates greater potential than nephrin for monitoring IMN severity.
  • MMP-9 may serve as a valuable indicator of kidney function in IMN patients.

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