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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Inactivating Gene Expression with Antisense Modified Oligonucleotides.

Sidney Altman1, Carlos Angele-Martinez2

  • 1Yale University New Haven CT USA, Arizona State University, Tempe AZUSA.

Acta Naturae
|October 28, 2021
PubMed
Summary
This summary is machine-generated.

Modified nucleotides effectively inactivate bacterial gene expression, targeting genes like Gyr A in organisms such as Plasmodium falciparum. This approach shows promise for treating plant and human infections.

Keywords:
Plasmodium falciparumbacteriacitrus plantsgene expressionmodified nucleotides

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Area of Science:

  • Molecular Biology
  • Antimicrobial Research
  • Oligonucleotide Therapeutics

Background:

  • Modified nucleotides, such as phosphoramidates and mesyl nucleotides, are known to effectively inhibit gene expression.
  • The Gyr A gene is a validated target in various organisms, including bacteria and Plasmodium falciparum.

Purpose of the Study:

  • To investigate the efficacy of modified nucleotides in gene expression inactivation.
  • To explore the potential of antisense reactions for treating bacterial infections in citrus plants.
  • To assess the susceptibility of human tissue culture cells to mesyl oligonucleotides.

Main Methods:

  • Gene expression analysis using modified nucleotides (phosphoramidates, mesyl nucleotides).
  • Antisense reaction assays in bacterial models relevant to citrus plant infections.
  • Assays on human tissue culture cells targeting specific genes.

Main Results:

  • Modified nucleotides demonstrated high effectiveness in inactivating bacterial gene expression.
  • Antisense reactions showed promise but were not fully effective against bacteria infecting citrus plants.
  • Human tissue culture cells were susceptible to mesyl oligonucleotides when targeting a different gene.

Conclusions:

  • Modified nucleotides represent a potent strategy for gene expression inactivation in bacteria.
  • Further research is needed to optimize antisense reactions for agricultural applications.
  • Mesyl oligonucleotides have potential therapeutic applications in human medicine.