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Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
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Imaging leukocyte migration through afferent lymphatics.

Victor Collado-Diaz1, Jessica D Medina-Sanchez1, Anastasia-Olga Gkountidi1

  • 1Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland.

Immunological Reviews
|October 28, 2021
PubMed
Summary
This summary is machine-generated.

Afferent lymphatic vessels transport immune cells like dendritic cells (DCs) and T cells to lymph nodes. Recent imaging reveals these cells actively migrate and interact within lymphatic capillaries, influencing immune responses.

Keywords:
T cellsTraffickingafferent lymphaticsdendritic cellsex vivo imagingin vivo imaginglymphatic endothelial cellsmigration

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Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • Afferent lymphatic vessels are crucial for transporting antigens and leukocytes, including dendritic cells (DCs) and T cells, from peripheral tissues to draining lymph nodes (dLNs).
  • These vessels play a significant role in initiating and regulating adaptive immunity.
  • Understanding leukocyte trafficking within these vessels is key to comprehending immune system function.

Purpose of the Study:

  • To review the biology of afferent lymphatic vessels.
  • To discuss the significance of DC and T cell migration through afferent lymphatics.
  • To highlight the impact of time-lapse imaging on understanding lymphatic trafficking and immune modulation.

Main Methods:

  • Review of existing literature and time-lapse imaging studies.
  • Analysis of in vivo and tissue explant imaging data.
  • Focus on cellular resolution visualization of leukocyte migration.

Main Results:

  • Leukocytes, particularly DCs and T cells, actively migrate and interact within lymphatic capillaries due to low flow conditions.
  • Cells spend considerable time migrating, patrolling, and interacting with lymphatic endothelium and other leukocytes.
  • Leukocyte detachment and lymph flow are enhanced in downstream collecting vessels due to contractions.

Conclusions:

  • Afferent lymphatics are vital for immune cell transport and play a role in immune modulation beyond simple transport.
  • Time-lapse imaging has significantly advanced the understanding of leukocyte trafficking within lymphatic capillaries.
  • Lymphatic capillaries are increasingly recognized as active sites of immune cell interaction and regulation.