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Updated: Oct 15, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MiRNA-375 inhibits retinoblastoma progression through targeting ERBB2 and inhibiting MAPK1/MAPK3 signalling pathway
Lei Liu1, Chunlin Xiao2, Qiuyun Sun3
1Department of Fundus Disease, Aier Eye Hospital Chongqing Children's, Chongqing City, China.
Background:
Increasing evidence has shown that the dysregulation of miRNAs is involved in the pathogenesis of retinoblastoma (RB). This present study was aimed to investigate the significance of miR-375 in RB progression, and the underlying mechanism.
Materials And Methods:
The miR-375 expression was detected by RT-PCR. CCK-8 assay and transwell assays were used to measure RB cell viability, migration, and invasion. The downstream gene of miR-375 was verified by luciferase reporter assay. Western blot was applied to detect the related proteins of MAPK1/MAPK3 signalling pathway.
Results:
MiR-375 was decreased significantly in RB tissues, and its down-regulation was associated with the poor prognosis of RB patients. Over-expression of miR-375 inhibited RB cell proliferation, migration, and invasion. More importantly, miR-375 modulated ERBB2 expression negatively, and ERBB2 was confirmed as the target of miR-375. Moreover, ERBB2 overturned the inhibitory effect of miR-375 mimic on the progression of RB. MiR-375 mimic suppressed RB progression via inhibiting the activation of MAPK1/MAPK3 signalling pathway.
Conclusions:
MiR-375 inhibited RB progression through targeting ERBB2 and suppressing MAPK1/MAPK3 signalling pathway, which might be a new target for the clinical treatment strategy.
Insights
MicroRNA-375 (miR-375) is significantly downregulated in retinoblastoma (RB), inhibiting tumor progression by targeting ERBB2 and suppressing the MAPK signaling pathway. This suggests miR-375 as a potential therapeutic target for RB.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA (miRNA) dysregulation is implicated in retinoblastoma (RB) pathogenesis.
- The specific role and mechanism of miR-375 in RB require further elucidation.
Purpose of the Study:
- To investigate the significance of miR-375 in RB progression.
- To elucidate the underlying molecular mechanism of miR-375 in RB.
Main Methods:
- Quantitative real-time PCR (RT-PCR) for miR-375 expression.
- Cell Counting Kit-8 (CCK-8) and Transwell assays for cell viability, migration, and invasion.
- Luciferase reporter assays and Western blotting to identify targets and pathways (MAPK1/MAPK3).
Main Results:
- MiR-375 expression was significantly decreased in RB tissues, correlating with poor prognosis.
- Overexpression of miR-375 suppressed RB cell proliferation, migration, and invasion.
- MiR-375 directly targeted ERBB2, inhibiting RB progression by suppressing the MAPK1/MAPK3 signaling pathway.
Conclusions:
- MiR-375 inhibits RB progression by targeting ERBB2 and downregulating the MAPK1/MAPK3 signaling pathway.
- MiR-375 represents a potential novel therapeutic target for retinoblastoma treatment.
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