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Genetically engineered mice for combinatorial cardiovascular optobiology.

Frank K Lee1, Jane C Lee1, Bo Shui1

  • 1Department of Biomedical Sciences, Cornell University, Ithaca, United States.

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Summary
This summary is machine-generated.

Researchers developed 21 new mouse lines for optogenetics, enabling real-time study of cellular signaling in cardiovascular biology. These genetically compatible tools allow direct combination for advanced in vivo research.

Keywords:
calcium imagingcell biologyimagingmouseoptogenetics

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Area of Science:

  • Physiology
  • Genetics
  • Biotechnology

Background:

  • Optogenetics offers real-time insights into physiological processes and molecular signaling.
  • Combining optogenetic tools in mice requires optically compatible and genetically tractable lines.
  • Existing methods often rely on Cre recombinase, necessitating multiallelic combinations.

Purpose of the Study:

  • To create a versatile toolbox of 21 mouse lines for lineage-specific optogenetic effector and sensor expression.
  • To enable direct biallelic combination of optogenetic tools, bypassing Cre-mediated recombination.
  • To focus on cardiovascular biology models for exploring cellular signaling in vivo.

Main Methods:

  • Developed 21 mouse lines with selective expression of optogenetic effectors (11 lines) and Ca2+ sensors (10 lines).
  • Targeted expression in cardiac pacemaker cells, cardiomyocytes, vascular cells, and other cell types.
  • Demonstrated functionality through optical modulation of arterial tone and cardiac activation, and combined use in biallelic crosses.

Main Results:

  • Optogenetic tools successfully modulated arterial/arteriolar tone via second messengers (InsP3, cAMP) and Ca2+ channels.
  • Cardiac function was controlled through optical activation of nodal/conducting cells and cardiomyocytes.
  • Achieved combined optogenetic effector and sensor function in biallelic crosses for optical cardiac pacing and cardiomyocyte Ca2+ imaging.

Conclusions:

  • The new mouse lines provide a powerful, combinable platform for studying cellular signaling in complex physiological systems.
  • This toolbox facilitates in vivo investigation of cardiovascular and other biological processes.
  • Enables advanced research by allowing simultaneous optical control and monitoring of cellular functions.