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SLCO4A1-AS1 Facilitates the Malignant Phenotype via miR-149-5p/STAT3 Axis in Gastric Cancer Cells

Qing Li1, Dachuan Zhang1, Hui Wang1

  • 1Department of Pathology, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Journal of Oncology
|October 29, 2021
PubMed

Insights

Newly discovered long non-coding RNA, SLCO4A1-AS1, promotes gastric cancer progression by targeting miR-149-5p and upregulating STAT3. This finding reveals a novel molecular mechanism in gastric cancer development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer remains a significant global health challenge.
  • The role of long non-coding RNAs (lncRNAs) in tumorigenesis is increasingly recognized.
  • The specific function of Solute carrier organic anion transporter family member 4A1 (SLCO4A1-AS1) in gastric cancer is largely unknown.

Purpose of the Study:

  • To elucidate the underlying molecular mechanism of SLCO4A1-AS1 in gastric cancer.
  • To investigate the interplay between SLCO4A1-AS1, miR-149-5p, and STAT3 in gastric cancer.
  • To assess the functional role of SLCO4A1-AS1 in gastric cancer cell behavior.

Main Methods:

  • Bioinformatics analysis, dual luciferase reporter assays, and Pearson correlation analysis to determine gene interactions.
  • Quantitative real-time PCR and Western blot to measure gene and protein expression levels.
  • Cell Counting Kit-8 (CCK-8), flow cytometry, wound healing, and Transwell assays to evaluate cellular functions; rescue experiments were performed.

Main Results:

  • SLCO4A1-AS1 and STAT3 expression were upregulated, while miR-149-5p was downregulated in gastric cancer tissues and cell lines.
  • SLCO4A1-AS1 positively correlated with STAT3 expression and negatively correlated with miR-149-5p expression.
  • Overexpression of SLCO4A1-AS1 enhanced cell viability, migration, and invasion, suppressed apoptosis, and increased STAT3 expression. Conversely, knockdown of SLCO4A1-AS1 showed opposite effects.
  • SLCO4A1-AS1 directly targets miR-149-5p, which in turn targets STAT3. Overexpression of miR-149-5p reversed the oncogenic effects of SLCO4A1-AS1.

Conclusions:

  • Abnormally elevated SLCO4A1-AS1 expression is implicated in the development of gastric cancer.
  • The SLCO4A1-AS1/miR-149-5p/STAT3 axis represents a critical molecular mechanism driving gastric cancer progression.
  • SLCO4A1-AS1 may serve as a potential therapeutic target for gastric cancer treatment.

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