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Related Experiment Video

Updated: Oct 15, 2025

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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High throughput screening data for a case study of CHO cell culture process development.

Qin He1, Jianlin Xu1, Erik Vandermark1

  • 1Biologics Development, Global Product Development and Supply, Bristol Myers Squibb, Devens, MA 01434, USA.

Data in Brief
|October 29, 2021
PubMed
Summary

High-throughput screening identified rosmarinic acid (RA) in chemically defined media to double monoclonal antibody (mAb) titer. This method systematically optimizes cell culture media for biopharmaceutical production.

Keywords:
CHO cell cultureHigh-throughput screeningMonoclonal antibody titerRosmarinic acid

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Area of Science:

  • Biotechnology
  • Cell Culture
  • Bioprocess Engineering

Background:

  • Complex hydrolysate media were historically used for monoclonal antibody (mAb) production in Chinese hamster ovary (CHO) cells.
  • Replacing hydrolysate media with chemically defined media is crucial for late-phase process development and regulatory compliance.
  • Optimizing media composition is key to enhancing mAb titer and production efficiency.

Purpose of the Study:

  • To identify an optimal chemically defined media formulation for late-phase process development.
  • To replace complex hydrolysate media with a chemically defined alternative for mAb production.
  • To improve mAb titer through systematic media optimization.

Main Methods:

  • Conducted four high-throughput screening (HTS) studies evaluating over 150 chemically defined media combinations.
  • Employed design of experiment (DOE), one-factor-at-a-time (OFAT), and definitive screening designs for media optimization.
  • Investigated the impact of basal media, feed media, and supplements like rosmarinic acid (RA) on mAb titer.

Main Results:

  • Achieved a two-fold increase in mAb titer compared to the early-phase process.
  • Identified rosmarinic acid (RA) as a key supplement for enhancing titer in chemically defined media.
  • Demonstrated the effectiveness of HTS in systematically determining optimal media compositions.

Conclusions:

  • Chemically defined media supplemented with rosmarinic acid (RA) significantly improves mAb titer in CHO cell cultures.
  • High-throughput screening provides a robust and statistically sound approach for media development.
  • This study offers a data-driven strategy for optimizing bioprocess media for enhanced therapeutic protein production.