Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Forced Transdifferentiation01:28

Forced Transdifferentiation

2.0K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
2.0K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reproducible Human Neural Circuits Printed with Single-Cell Precision Reveal the Functional Roles of Ephaptic Coupling.

ACS nano·2025
Same author

A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging.

bioRxiv : the preprint server for biology·2025
Same author

Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

hafoe: an interactive tool for the analysis of chimeric AAV libraries after random mutagenesis.

Gene therapy·2025
Same author

Medical digital twins: enabling precision medicine and medical artificial intelligence.

The Lancet. Digital health·2025
Same author

Aging on Chip: Harnessing the Potential of Microfluidic Technologies in Aging and Rejuvenation Research.

Advanced healthcare materials·2025

Related Experiment Video

Updated: Oct 15, 2025

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

5.8K

Neuronal Cell-type Engineering by Transcriptional Activation.

Songlei Liu1,2, Johannes Striebel3, Giovanni Pasquini3

  • 1Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, United States.

Frontiers in Genome Editing
|October 29, 2021
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas gene activation offers powerful tools for studying gene function and disease. This review highlights CRISPR-based strategies for neuronal differentiation and nervous system applications.

Keywords:
CRISPRaORFforward programmingneurontranscription factor

More Related Videos

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

7.9K
Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases
09:44

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Published on: May 2, 2025

373

Related Experiment Videos

Last Updated: Oct 15, 2025

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

5.8K
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

7.9K
Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases
09:44

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Published on: May 2, 2025

373

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Gene Editing Technologies

Background:

  • The CRISPR-Cas system enables precise gene activation, crucial for understanding gene function and disease.
  • Targeted gene activation is vital for controlling cellular behavior and disease progression.
  • Neuronal differentiation is a key area for studying neurological disorders and developing therapies.

Purpose of the Study:

  • To review recent advancements in CRISPR-Cas transcriptional activation (CRISPRa) and open reading frame (ORF) expression for targeted gene activation.
  • To discuss the technical parameters of CRISPRa and ORF-based strategies for neuronal programming.
  • To highlight in vivo applications of CRISPRa in the nervous system.

Main Methods:

  • Survey of recent studies utilizing CRISPRa and ORF expression for gene activation.
  • Analysis of technical parameters for neuronal programming strategies.
  • Review of in vivo studies applying CRISPRa to the nervous system.

Main Results:

  • CRISPRa and ORF expression are effective for targeted gene activation and multiplexed screening.
  • CRISPRa-based strategies show promise for inducing neuronal differentiation.
  • In vivo applications of CRISPRa in the nervous system are expanding.

Conclusions:

  • CRISPRa is a valuable tool for neuronal cell-type programming.
  • CRISPRa-based methods offer new avenues for neuroscience research and therapeutic development.
  • Further research into CRISPRa applications will advance our understanding of the nervous system.