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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
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Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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[Anti-interference hemoglobin analysis system by high performance liquid chromatography].

Yan Xu1, Tiantian Yao2, Wenyong Hu2

  • 1College of Bioengineering, Chongqing University, Chongqing 400044, P.R.China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|October 29, 2021
PubMed
Summary

A new HPLC system accurately detects glycated hemoglobin (HbA1c) and thalassemia factors (HbF/HbA2), overcoming interference issues. This stable, anti-interference system offers rapid screening for diabetes and hemoglobin disorders.

Keywords:
glycated hemoglobinhemoglobin varianthigh performance liquid chromatographythalassemia factor HbF/HbA2

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Area of Science:

  • Analytical Chemistry
  • Clinical Diagnostics
  • Biochemistry

Background:

  • High-performance liquid chromatography (HPLC) is standard for hemoglobin detection.
  • Glycated hemoglobin (HbA1c) is crucial for diabetes diagnosis.
  • HPLC accuracy for HbA1c is compromised by thalassemia factors (HbF/HbA2) and variant hemoglobins.

Purpose of the Study:

  • To develop a novel HPLC system for anti-interference hemoglobin analysis.
  • To simultaneously detect HbA1c, HbF, HbA2, and variant hemoglobins.
  • To improve the accuracy and stability of hemoglobin testing.

Main Methods:

  • Optimization of a high-pressure three-gradient elution method.
  • Modification of chromatography column particle size and sieve plate aperture.
  • Enhancement of the double-plunger reciprocating series high-pressure pump structure.

Main Results:

  • The new system demonstrated anti-interference and stable performance.
  • It accurately diagnosed HbA1c, HbF/HbA2, and variant hemoglobins.
  • Excellent linear correlation was observed when compared to leading global HPLC systems.

Conclusions:

  • The developed HPLC system effectively screens for HbA1c and thalassemia factors.
  • This represents the first domestic HPLC system for rapid and accurate screening of HbA1c and thalassemia factors.
  • The system shows potential for industrialization and improved clinical diagnostics.