Increasing sulfate concentration and sedimentary decaying cyanobacteria co-affect organic carbon mineralization in...

Sulfate (SO₄^2-) concentrations in eutrophic lakes are continuously increasing; however, the effect of increasing SO₄^2- concentrations on organic carbon mineralization, especially the greenhouse gas emissions of sediments, remains unclear. Here, we constructed a series of microcosms with initial SO₄^2- concentrations of 0, 30, 60, 90, 120, 150, and 180 mg/L to study the effects of increased SO₄^2- concentrations, coupled with cyanobacterial blooms, on organic carbon mineralization in Lake Taihu. Cyanobacterial blooms promoted sulfate reduction and released a large amount of inorganic carbon. The SO₄^2- concentrations in cyanobacteria treatments significantly decreased and eventually reached close to 0. As the initial SO₄^2- concentration increased, the sulfate reduction rates significantly increased, with maximum values of 9.39, 9.44, 28.02, 30.89, 39.68, and 54.28 mg/L∙d for 30, 60, 90, 120, 150, and 180 mg/L SO₄^2-, respectively. The total organic carbon content in sediments (51.16-52.70 g/kg) decreased with the initial SO₄^2- concentration (R² = 0.97), and the total inorganic carbon content in overlying water (159.97-182.73 mg/L) showed the opposite pattern (R² = 0.91). The initial SO₄^2- concentration was positively correlated with carbon dioxide (CO₂) emissions (R² = 0.68) and negatively correlated with methane (CH₄) emissions (R² = 0.96). The highest CO₂ concentration and lowest CH₄ concentration in the 180 mg/L SO₄^2- treatment were 1688.78 and 1903 μmol/L, respectively. These biogeochemical processes were related to competition for organic carbon sources between sulfate reduction bacteria (SRB) and methane production archaea (MPA) in sediments. The abundance of SRB was positively correlated with the initial SO₄^2- concentration and ranged from 6.65 × 10⁷ to 2.98 × 10⁸ copies/g; the abundance of MPA showed the opposite pattern and ranged from 1.99 × 10⁸ to 3.35 × 10⁸copies/g. These findings enhance our understanding of the effect of increasing SO₄^2- concentrations on organic carbon mineralization and could enhance the accuracy of assessments of greenhouse gas emissions in eutrophic lakes.