Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.5K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alternate RNA decoding results in stable and abundant proteins in mammals.

Nature·2026
Same author

Viral protease-mediated polyprotein processing in human astroviruses.

Npj viruses·2026
Same author

Efficient Plasmid-Based Rescue of T7 RNA Polymerase-Driven Calicivirus Reverse Genetics Systems in Mammalian Cells Using Vaccinia Virus RNA-Capping Enzymes.

Viruses·2026
Same author

Fertilization triggers early proteomic symmetry breaking in mammalian embryos.

Cell·2025
Same author

Principles of protein abundance regulation across single cells in a mammalian tissue.

bioRxiv : the preprint server for biology·2025
Same author

Slice-PASEF: Maximising Ion Utilisation in LC-MS Proteomics.

bioRxiv : the preprint server for biology·2025
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026
Same journal

Facilitating structure-based drug discovery with an artificial intelligence-driven virtual screening platform.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Oct 15, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

4.1K

Multiplexed single-cell proteomics using SCoPE2.

Aleksandra A Petelski1,2, Edward Emmott1,2,3, Andrew Leduc1,2

  • 1Department of Bioengineering, Northeastern University, Boston, MA, USA.

Nature Protocols
|October 30, 2021
PubMed
Summary
This summary is machine-generated.

The Single Cell ProtEomics (SCoPE2) protocol enables cost-effective, automated, and scalable single-cell protein quantification using mass spectrometry. This method enhances peptide identification and specificity for analyzing diverse biological systems.

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.8K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.2K

Related Experiment Videos

Last Updated: Oct 15, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

4.1K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.8K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.2K

Area of Science:

  • Proteomics
  • Cellular Biology
  • Biotechnology

Background:

  • Biological systems exhibit cellular diversity requiring advanced analysis.
  • Traditional single-cell protein analysis relies on affinity reagents.
  • Mass spectrometry offers enhanced specificity and quantification of cellular proteins.

Purpose of the Study:

  • Introduce the Single Cell ProtEomics (SCoPE2) protocol.
  • Enable high-throughput, cost-effective single-cell protein quantification.
  • Improve peptide identification in single-cell proteomic analysis.

Main Methods:

  • Single-cell isolation via FACS or CellenONE.
  • Peptide lysis using Minimal ProteOmic sample Preparation (mPOP).
  • Isobaric labeling with TMT or TMTpro for multiplexed mass spectrometry.

Main Results:

  • SCoPE2 enhances peptide sequence identification using an isobaric carrier.
  • Achieved cost-effective, automated, and scalable single-cell protein quantification.
  • Workflow analyzes ~200 single cells per 24h with standard equipment.

Conclusions:

  • SCoPE2 provides a robust and scalable method for single-cell protein analysis.
  • The protocol is cost-effective, automatable, and applicable to various samples.
  • SCoPE2 advances functional and molecular insights into cellular diversity.