Related Experiment Video

Updated: Oct 15, 2025

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.3K

Structure, regulation, and host interaction of outer membrane protein U (OmpU) of Vibrio species

Hilal A Ganie1, Aaina Choudhary1, Somesh Baranwal1

  • 1Department of Microbiology, School of Basic Science, Central University of Punjab, VPO Ghudda, Bathinda, Punjab, 151401 , India.

Microbial Pathogenesis
|October 31, 2021
PubMed

Insights

Outer membrane protein U (OmpU) from Vibrio species is a key adhesion and virulence factor. It interacts with host cells, triggering immune responses and promoting bacterial colonization, with potential applications in aquaculture immunity.

Area of Science:

  • Microbiology
  • Immunology
  • Structural Biology

Background:

  • OmpU is an outer membrane protein found in Vibrio species.
  • It non-covalently interacts with the peptidoglycan layer.
  • OmpU plays a role in bacterial virulence and host interactions.

Purpose of the Study:

  • To review the structural, regulatory, and functional mechanisms of OmpU.
  • To explore OmpU's role in host-pathogen interactions.
  • To discuss OmpU's potential in aquaculture immunity.

Main Methods:

  • Literature review of existing studies on OmpU.
  • Analysis of OmpU's interaction with host cells and immune systems.
  • Examination of in-vivo studies using recombinant OmpU and mimotopes.

Main Results:

  • OmpU triggers host cell signaling, cytokine secretion, ROS production, and caspase-independent death.
  • It confers resistance to antimicrobial peptides and reorganizes host actin cytoskeleton.
  • OmpU elicits immune responses in aquaculture hosts, providing short-term protection.

Conclusions:

  • OmpU is a crucial adhesion protein and virulence factor for Vibrio colonization.
  • Understanding OmpU's mechanisms offers insights into disease states and potential therapeutic strategies.
  • OmpU-based approaches show promise for enhancing immunity in aquaculture.
Keywords:
Disease progressionInnate immunityNFκBVaccineVirulence

More Related Videos

A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development11:17

A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

Published on: June 7, 2012

23.6K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

655

Related Concept Videos

Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
173
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
261
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
159
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
227
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
173
Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
489