Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

3.8K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
3.8K
Proteomics01:33

Proteomics

8.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The clinical and molecular landscape of thalamic glioma.

Neuro-oncology·2026
Same author

Human Hematopoietic Stem Cells Enhance Maturational Differentiation of hiPSC-Derived Cardiomyocytes on Xeno-Free MatriClone-Plastic via EGFR/MAPK/ERK Signaling Pathway.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Selective blockade of γc pathway ameliorates alopecia areata in a humanized murine model.

The Journal of investigative dermatology·2026
Same author

Base editing of Artemis mutations ex vivo sheds light on gene therapy for Artemis-deficient SCID.

Advanced biotechnology·2026
Same author

Amphioxus tyrosine kinase SYK inhibits NF-κB activation through MyD88-dependent mechanism.

Journal of molecular cell biology·2026
Same author

CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylation.

Nucleic acids research·2026

Related Experiment Video

Updated: Oct 15, 2025

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

13.9K

Complement system deregulation in SAPHO syndrome revealed by proteomic profiling.

Yuxiu Sun1, Chen Li2, Wanchen Yu1

  • 1School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.

Journal of Proteomics
|October 31, 2021
PubMed
Summary

This study identifies key proteins in SAPHO syndrome, a rare inflammatory disease. Complement system inhibitors, CFH and C4BP, show potential as diagnostic biomarkers for SAPHO syndrome.

Keywords:
Complement systemInhibitionPlasmaProteomic profileSAPHO syndrome

More Related Videos

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

15.7K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.9K

Related Experiment Videos

Last Updated: Oct 15, 2025

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

13.9K
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

15.7K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.9K

Area of Science:

  • Immunology
  • Proteomics
  • Rheumatology

Background:

  • SAPHO (Synovitis, Acne, Pustulosis, Hyperostosis, Osteitis) syndrome is a rare inflammatory disorder affecting skin and bone.
  • Diagnosis is challenging due to low incidence and diverse clinical presentations.
  • Identifying reliable biomarkers is crucial for early diagnosis and understanding SAPHO pathogenesis.

Purpose of the Study:

  • To investigate the serum proteomic profile of SAPHO syndrome patients.
  • To identify differentially expressed proteins (DEPs) as potential biomarkers for SAPHO.
  • To explore the role of identified proteins in SAPHO pathogenesis.

Main Methods:

  • Serum samples from 8 SAPHO patients and 8 healthy controls were analyzed using data-independent acquisition (DIA) mass spectrometry.
  • Differentially expressed proteins (DEPs) were identified using statistical analysis (p < 0.05, fold change > 1.2).
  • Overexpression of complement inhibitors (CFH, C4BP) was validated in a larger cohort (16 SAPHO, 8 AS, 24 controls) using ELISA and ROC curve analysis.

Main Results:

  • A total of 57 DEPs were identified, with 27 upregulated and 30 downregulated in SAPHO patients.
  • DEPs were associated with pathways including 'complement and coagulation cascades' and 'mTOR signaling pathway'.
  • Complement inhibitors CFH and C4BP were significantly overexpressed in SAPHO patients, achieving an AUC of 0.91 for combined diagnostic ability.

Conclusions:

  • This study provides the first proteomic insights into SAPHO syndrome plasma.
  • Elevated levels of complement inhibitors CFH and C4BP may serve as potential diagnostic biomarkers for SAPHO syndrome.
  • These findings suggest a role for the complement system in SAPHO pathogenesis.