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Complement component C3 secretion by mouse macrophage-like cell lines.

K J Goodrum

    Journal of Leukocyte Biology
    |April 1, 1987
    PubMed
    Summary
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    Mouse macrophage cell lines secrete complement component C3. Their C3 secretion is inducible by various stimuli but inhibited by cycloheximide or hydrocortisone, offering models for complement biosynthesis research.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Macrophages are key immune cells involved in innate and adaptive immunity.
    • Complement component C3 is a central protein in the complement system, crucial for immune responses.
    • Understanding C3 regulation in macrophages is vital for immune system research.

    Purpose of the Study:

    • To investigate C3 secretion by various mouse macrophage cell lines.
    • To determine the inducibility of C3 secretion by different immune stimuli.
    • To establish these cell lines as models for studying complement biosynthesis regulation.

    Main Methods:

    • Enzyme-linked immunosorbent assay (ELISA) was used to quantify mouse C3 secretion.
    • Four mouse macrophage-like cell lines (PU5-1.8, J774A.1, RAW264.7, P388D1) were cultured.

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  • Cells were treated with lipopolysaccharide, streptococcal cell walls, or lymphokines; C3 secretion was measured, along with controls like cycloheximide and hydrocortisone.

    • Main Results:

    • All tested macrophage cell lines secreted detectable levels of C3, with varying baseline secretion rates.
    • C3 secretion was significantly enhanced (2-4 fold) by lipopolysaccharide, streptococcal cell walls, and lymphokines.
    • Relative inducibility varied among cell lines, and C3 secretion was inhibited by cycloheximide and hydrocortisone.

    Conclusions:

    • Mouse macrophage cell lines possess both baseline and inducible C3 synthetic and secretory activities.
    • These cell lines serve as valuable, homogeneous models for investigating the regulation of complement biosynthesis.
    • Findings highlight the differential regulation of C3 synthesis and secretion compared to other proteins like lysozyme.