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Related Experiment Video

Updated: Oct 15, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

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High-Throughput CRISPR-Cas13 SARS-CoV-2 Test.

Brendan J Manning1, Wahab A Khan2,3, Jennifer M Peña1

  • 1R&D Department, Sherlock Biosciences, Boston, MA.

Clinical Chemistry
|October 31, 2021
PubMed
Summary
This summary is machine-generated.

A new CRISPR-based diagnostic test offers rapid, scalable detection of SARS-CoV-2. This high-throughput assay provides accurate COVID-19 results from saliva or swabs in under an hour.

Keywords:
infectious diseasemolecular diagnosticsviral diseases

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Area of Science:

  • Molecular Biology
  • Infectious Disease Diagnostics
  • Biotechnology

Background:

  • The COVID-19 pandemic highlighted the need for rapid, scalable, and easily deployable diagnostic solutions.
  • Current diagnostic methods often face limitations in speed, throughput, and ease of use, hindering effective disease control.

Purpose of the Study:

  • To develop and evaluate a novel diagnostic method for the detection of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2).
  • To achieve sensitive, specific, and high-throughput detection of SARS-CoV-2 RNA using CRISPR technology.

Main Methods:

  • Development of a CRISPR-based assay utilizing SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) for qualitative detection of SARS-CoV-2 RNA.
  • The assay was designed for direct use on swab or saliva samples, eliminating the need for nucleic acid extraction.
  • A 384-well format was employed to enable high-throughput analysis, with results deliverable in under one hour.

Main Results:

  • The high-throughput SHERLOCK SARS-CoV-2 assay demonstrated 100% concordance with CDC RT-qPCR methods when combined with magnetic bead-based extraction (n=60).
  • Direct sample addition in a high-throughput format also showed 100% concordance with the CDC direct RT-qPCR method (n=45).
  • With direct saliva addition, the assay achieved 100% negative and 88% positive percentage agreement compared to a clinical laboratory's results.

Conclusions:

  • The developed high-throughput CRISPR-based assay accurately identifies SARS-CoV-2 from patient samples, with or without nucleic acid extraction.
  • The assay shows high concordance with established RT-qPCR methods, offering a reliable alternative for diagnostics.
  • This test empowers high-complexity laboratories to significantly increase their testing capacity using simple equipment.