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Updated: Oct 15, 2025
07:45
Western Blotting: Sample Preparation to Detection
Published on: October 14, 2010
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Western Blot Processing Optimization: The Perfect Blot
Russ Yukhananov1, David P Chimento2, Laura A Marlow3
1Precision Biosystems LLC, Mansfield, MA, USA. ryyuk@precisionbiosystems.com.
Methods in Molecular Biology (Clifton, N.J.)
|October 31, 2021
Abstract:
Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electrophoresis separation, transfer to a membrane, and immunodetection with specific antibodies. Here, we show that optimization of washing helps to maximize the specific interactions of antigens and antibodies. Performing all washing steps at 4 °C ensures a maximal signal to noise ratio and reduces nonspecific signals.
Keywords:
Antibodies specificityAutomationElectrophoresisImmunodetectionWashing temperatureWestern blotMore Related Videos
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