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Published on: December 22, 2014
Construction of Metatranscriptomic Libraries for 5' End Sequencing of rRNAs for Microbiome Research
1Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland. mtiirola@jyu.fi.
Abstract:
Metatranscriptomic sequencing enables studying community-wide gene expression profiles of microbial samples and getting functional insight on their up- or downregulated pathways. However, shotgun sequencing is not the most efficient way to study expression of ribosomal RNA genes or to compare lot of samples in experimental setups. Here we describe an efficient primer-independent method for processing and barcoding libraries for directional sequencing of the 5' end region of the RNA. When applying size selection of the original RNA, the method forms an optimal solution for the simultaneous analysis of bacterial, archaeal, and eukaryotic rRNA diversity.
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