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Updated: Oct 14, 2025

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

A Humanized Mouse Strain That Develops Spontaneously Immune-Mediated Diabetes.

Sandrine Luce^1,2, Sophie Guinoiseau^1,2, Alexis Gadault^1,2

¹Laboratory Immunology of Diabetes, INSERMU1016, Department EMD, Cochin Institute, Paris, France.

Frontiers in Immunology

|November 1, 2021

Summary

A novel humanized mouse model, YES-RIP-hB7.1, spontaneously develops type 1 diabetes (T1D) with human T-cell responses. This model aids in understanding T1D pathogenesis and evaluating immunotherapies for patients.

Keywords:

HLA-DQ8 autoimmunity epitopes humanized mouse preproinsulin type 1 diabetes (T1D)

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Published on: August 16, 2019

Area of Science:

Immunology
Endocrinology
Genetics

Background:

Preclinical models for type 1 diabetes (T1D) have limitations in fully recapitulating human disease.
Understanding the autoimmune response to insulin-secreting cells is crucial for T1D research.

Purpose of the Study:

To develop a new humanized mouse model to study T1D pathogenesis.
To investigate human T-cell responses to insulin-related epitopes in a T1D context.
To provide a platform for evaluating T1D immunotherapies.

Main Methods:

Development of the YES-RIP-hB7.1 mouse model, deficient in murine MHC and insulin genes, expressing human HLA alleles, insulin, and B7.1 in pancreatic beta cells.
Induction of spontaneous T1D and analysis of CD4+ and CD8+ T-cell responses to human preproinsulin epitopes.
Validation of observed T-cell responses in human T1D patients.

Main Results:

The YES-RIP-hB7.1 mouse model develops spontaneous T1D.
CD4+ and CD8+ T-cell responses targeting human preproinsulin epitopes were observed in these mice.
A significant portion of the identified T-cell responses were also found in human T1D patients.

Conclusions:

The YES-RIP-hB7.1 mouse is a valuable humanized model for studying T1D.
This model facilitates the characterization of T1D-associated epitopes and triggers of autoimmunity.
It offers a preclinical platform for testing peptide-based immunotherapies relevant to human T1D heterogeneity.