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A needle tip CCEA microfluidic device based on enhanced Dean flow for cell washing.

Xin Shi1, Wei Tan1, Yuwen Lu1

  • 1School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350 China.

Microsystems & Nanoengineering
|November 1, 2021
PubMed
Summary

A novel needle-tip microdevice using a circular contraction-expansion array (CCEA) enables efficient particle and cell washing with reduced sample loss. This technology offers high efficiency and cell viability for biological and clinical applications.

Keywords:
ChemistryNanoscale devices

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Particle and cell washing are crucial in biological and clinical settings.
  • Existing methods can suffer from sample loss and require complex connections.

Purpose of the Study:

  • To introduce a novel needle-tip microfluidic device for efficient particle and cell washing.
  • To evaluate the performance of the circular contraction-expansion array (CCEA) microdevice.

Main Methods:

  • Computational fluid dynamics (CFD) simulations were used to analyze fluid flow.
  • Experimental washing of particles (10-20 μm) and cell suspensions (varying concentrations) was performed.
  • Device performance was assessed based on focusing speed, particle/cell loss rate, collection efficiency, and cell viability.

Main Results:

  • The CCEA structure demonstrated enhanced Dean flow for faster particle/cell focusing compared to other designs.
  • Optimal flow rates (1:3 ratio, 120 μL/min total) ensured stable fluid distribution and high focusing efficiency.
  • Particle loss was reduced from 64% to 7% for rare samples, and cell collection efficiency exceeded 85% for three cell lines.
  • High cell activity (>93%) was maintained after washing.

Conclusions:

  • The needle-tip CCEA microfluidic device offers a compact, stable, and efficient solution for particle and cell washing.
  • It significantly reduces sample loss and maintains cell viability, showing potential for basic medical research and clinical diagnosis.