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Isolation and Characterization of Microvesicles from Peripheral Blood
Published on: January 6, 2017
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Isolation of Microvesicles from Human Circulating Neutrophils
Dong Zhan1, Ellie McConachie2, Steven W Edwards3
1Department of Biochemistry, Osteoarthritis and Musculoskeleton Research Unit, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
Bio-Protocol
|November 1, 2021
Summary
Neutrophil-derived microvesicles (NDMVs) are anti-inflammatory and protect cartilage in arthritis. This study details a method for isolating these potent NDMVs from human blood for therapeutic research.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Neutrophils are key players in acute inflammation.
- Neutrophil-derived microvesicles (NDMVs) possess anti-inflammatory properties.
- NDMVs demonstrate protective effects on cartilage in inflammatory arthritis models.
Purpose of the Study:
- To describe a reliable protocol for isolating neutrophil-derived microvesicles (NDMVs).
- To facilitate further research into the therapeutic potential of NDMVs for inflammatory diseases.
Main Methods:
- Isolation of neutrophils from whole human blood via differential centrifugation.
- Identification of neutrophils using cytospin and Wright's staining.
- Separation of NDMVs from neutrophils using differential centrifugation.
Main Results:
- A reproducible method for NDMV isolation was established.
- The protocol allows for the collection of functional NDMVs.
Conclusions:
- NDMVs hold significant potential as disease-modifying agents for inflammatory conditions.
- The described isolation protocol provides a foundation for NDMV-based therapeutic strategies.
