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Updated: Oct 14, 2025
A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
Identification and characterization of proteins, lipids, and metabolites in two organic fertilizer products derived
Jianyu Li1, Xin Zhao1, Laura S Bailey2
1Horticultural Sciences Department, University of Florida, Gainesville, FL 32611-0690 USA.
Abstract:
The biochemical composition of organic fertilizers largely determines their nutrient supply characteristics following soil application as well as their potential impact on soil microbial communities. Yet, limited information is available regarding the biochemical composition of organic fertilizers derived from different nutrient sources. Here, we qualitatively analyzed the presence and abundance of proteins, lipids, and metabolites in a liquid fish fertilizer (LFF) product and a type of granular organic fertilizer (GOF) commonly used in organic vegetable production, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our results suggest that the presence and abundance of proteins, lipids, and metabolites differ greatly between GOF and LFF. The qualitative analysis shows LFF as a rich source of metabolites, while complex proteins and long-chain saturated fatty acids are dominant in GOF. The degree of biochemical composition complexity may help explain the varying impacts of different types of organic fertilizers on nutrient availability, soil health, and environmental quality.
Supplementary Information:
The online version contains supplementary material available at 10.1186/s13765-021-00625-2.
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