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Updated: Oct 14, 2025
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Published on: May 21, 2018
Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
Xingchen Dong1, Lin-Feng Chen1,2
1Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Abstract:
NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).
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