Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Four in One: Parallel Determination of Optical Activity and Optical Anisotropy from Single Plasmonic Nanostructures.

ACS nano·2026
Same author

Heterogeneous Reactivity of Palladium Nanoparticles Revealed by Wavelength-Resolved Interferometric Scattering.

Nano letters·2026
Same author

Solvated Electron Generation from Coupled Plasmon Modes of Gold Nanoparticles Using Visible Light.

Nano letters·2026
Same author

Resolving Single-Particle Absorption and Scattering by Plasmonic Magnesium Nanoparticles.

Nano letters·2026
Same author

Peer Review and AI: Your (Human) Opinion Is What Matters.

ACS nano·2026
Same author

Single-Particle Emission Microscopy of Green-Emitting Carbon Dots Made from Top-Down and Bottom-Up Precursors.

The journal of physical chemistry letters·2025

Related Experiment Video

Updated: Oct 14, 2025

NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

204.2K

Toward Quantitative Nanothermometry Using Single-Molecule Counting.

Phillip A Reinhardt1, Abigail P Crawford1, Claire A West2

  • 1Department of Chemistry, Temple University, Philadelphia, Pennsylvania 19122, United States.

The Journal of Physical Chemistry. B
|November 1, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a DNA nanothermometry technique to measure single nanoparticle surface temperatures. This method uses DNA denaturation to quantify heat, advancing applications in nanomedicine and materials science.

More Related Videos

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering
07:53

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Published on: August 6, 2021

2.3K
Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
07:08

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Published on: October 20, 2020

7.6K

Related Experiment Videos

Last Updated: Oct 14, 2025

NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

204.2K
Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering
07:53

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Published on: August 6, 2021

2.3K
Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
07:08

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Published on: October 20, 2020

7.6K

Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Accurate temperature measurement at the nanoscale is crucial for various applications.
  • Existing methods lack the precision to determine surface temperature on single nanoparticles.
  • Photothermal heating of nanoparticles has broad applications, including nanomedicine and photocatalysis.

Purpose of the Study:

  • To develop a super-resolution DNA nanothermometry technique for measuring single plasmonic nanoparticle surface temperatures.
  • To establish a quantitative method for nanoscale temperature sensing.
  • To enable precise temperature measurements for improved control in nanoparticle applications.

Main Methods:

  • Functionalizing gold nanoparticles with double-stranded DNA.
  • Utilizing DNA denaturation as a temperature reporter.
  • Probing denatured DNA with fluorescently labeled DNA oligomers.
  • Reconstructing DNA melting curves by counting fluorescent binding events.

Main Results:

  • Demonstrated a DNA nanothermometry technique capable of reporting surface temperature on single plasmonic nanoparticles.
  • Reconstructed DNA melting curves that align with solution-phase DNA behavior.
  • Showcased control over denaturation temperature by adjusting Na+ concentration and DNA base pair length.
  • Achieved quantitative temperature measurements at nanoscale surfaces.

Conclusions:

  • The developed DNA nanothermometry technique provides a novel approach for precise nanoscale temperature sensing.
  • This method offers control over temperature probing windows, enabling quantitative surface temperature measurements.
  • The technique has significant potential for advancing fields reliant on precise nanoparticle thermal management, such as nanomedicine and photocatalysis.