Splanchnic oxygen saturation during reoxygenation with 21% or 100% O₂ in newborn piglets

Increasing evidence recognizes the harm of excess oxygen to lungs, eyes, and brain of preterm infants, but not yet to the intestine. We assessed changes in splanchnic oxygenation during reoxygenation with 21% compared to 100% O₂ in a newborn piglet model of perinatal asphyxia.

We randomized 25 piglets to control or intervention. Intervention groups underwent global hypoxia until acidosis and hypotension occurred. Piglets were reoxygenated for 30 min with 21% or 100% O₂ and observed for 9 h. We continuously measured regional splanchnic oxygen saturation (r_sSO₂) using near-infrared spectroscopy (NIRS). We calculated mean r_sSO₂ and r_sCoVar (as SD/mean). We measured PaO₂ and SaO₂, sampled from the right carotid artery.

Reoxygenation after global hypoxia restored r_sSO₂. Reoxygenation with 100% O₂ increased r_sSO₂ to values significantly higher than baseline. In intervention groups, r_sCoVar decreased during observation compared to baseline. We found a correlation between r_sSO₂ and PaO₂ (r = 0.420, P < 0.001) and between r_sSO₂ and SaO₂ (r = 0.648, P < 0.001) in pooled data from the entire experiment.

Reoxygenation after global hypoxia improves splanchnic oxygenation, but is associated with reduced variability of r_sSO₂. Reoxygenation with 100% O₂ exposes the intestine to hyperoxia. Splanchnic NIRS is able to detect intestinal hypoxia and hyperoxia.

Splanchnic oxygenation improves during reoxygenation after global hypoxia, though reoxygenation with 100% O₂ exposes the intestine to hyperoxia. Decreased variability of splanchnic oxygenation several hours after hypoxia and reoxygenation seems to be independent of the resuscitation strategy, and may indicate intestinal injury. Splanchnic NIRS monitoring was able to detect intestinal hypoxia and exposure to hyperoxia, as evidenced by a strong correlation between splanchnic oxygenation and arterial oxygen content.