Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

42.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
42.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Resolution Melting (HRM) of Hypervariable Mitochondrial DNA Regions for Forensic Science.

Journal of forensic sciences·2017
See all related articles

Related Experiment Video

Updated: Oct 14, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.0K

Touch DNA recovery from vehicle surfaces using different swabs.

Alexandre Giovanelli1, Rodrigo Grazinoli Garrido1, Alípio Rocha1

  • 1Departamento de Polícia Técnico-Científica do Rio de Janeiro, Instituto de Pesquisa e Perícia em Genética Forense, Rio de Janeiro, Brazil.

Journal of Forensic Sciences
|November 2, 2021
PubMed
Summary

The PurFlock® swab is most effective for collecting touch DNA from vehicle crime scenes, outperforming other swabs in DNA recovery and allele amplification, especially for small DNA amounts.

Keywords:
4N6FLOQSwabs™PurFlock® swabcotton swabtouch DNAtrace DNAvehicle surface

More Related Videos

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.4K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

21.3K

Related Experiment Videos

Last Updated: Oct 14, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.0K
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.4K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

21.3K

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Touch DNA collection is crucial in forensic investigations.
  • The efficiency of touch DNA recovery is influenced by collection devices and surfaces.
  • Evaluating different swab types is essential for optimizing forensic sample collection.

Purpose of the Study:

  • To compare the efficiency of three distinct swab types in recovering touch DNA from vehicle surfaces.
  • To determine the optimal swab for touch DNA collection in automotive forensic contexts.

Main Methods:

  • Three swab types (PurFlock®, 4N6FLOQSwabs™, cotton) were tested.
  • DNA was collected from the gearshift, parking brake, and steering wheel of a vehicle.
  • DNA yield and allele amplification rates were analyzed for each swab type.

Main Results:

  • Significant differences were observed in DNA recovery and allele amplification rates among the swabs (p < 0.05).
  • The PurFlock® swab demonstrated the highest DNA recovery efficiency, followed by cotton, then 4N6FLOQSwabs™.
  • No significant differences in DNA recovery were found across the analyzed vehicle surfaces.

Conclusions:

  • The PurFlock® swab is superior for touch DNA recovery from vehicles, particularly for low DNA quantity samples.
  • This study underscores the importance of selecting appropriate collection materials for forensic DNA analysis.
  • Further research should consider the interplay of collection, extraction, and amplification methods.