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Related Experiment Video

Updated: Oct 14, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Optofluidic ptychography on a chip.

Pengming Song¹, Chengfei Guo¹, Shaowei Jiang¹

¹Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA. chengfei.guo@uconn.edu.

|November 2, 2021

Summary

This summary is machine-generated.

We developed an on-chip, lensless microscopy technique called optofluidic ptychography. This method integrates high-resolution imaging into microfluidic devices for advanced biospecimen analysis.

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Area of Science:

Optofluidics
Microscopy
Biophotonics

Background:

Microfluidic devices offer miniaturization for lab-on-a-chip applications.
Ptychographic microscopy provides high-resolution quantitative imaging from diffraction data.
Integrating microscopy with microfluidics enables on-chip analysis of biological samples.

Purpose of the Study:

To implement a fully on-chip, lensless microscopy technique.
To integrate ptychographic microscopy with microfluidics for lab-on-a-chip devices.
To demonstrate high-resolution imaging of flowing biospecimens.

Main Methods:

A microfluidic channel was placed on a scattering layer-coated coverslip atop an image sensor.
Microfluidic flow delivered specimens through the channel.
Diffracted light was recorded for ptychographic reconstruction.

Main Results:

Achieved resolution of 550 nm linewidth using a 1.85 μm pixel size image sensor.
Successfully imaged various biospecimens including *C. elegans*, yeast, *paramecium*, and *closterium sp*.
Demonstrated high-resolution ptychographic reconstruction at 30 frames per second.

Conclusions:

Optofluidic ptychography enables on-chip, lensless, high-resolution imaging.
This technique complements microfluidics and integrates into lab-on-a-chip systems.
The method has broad biomedical applications and potential for flow cytometer configurations.