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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Related Experiment Video

Updated: Oct 14, 2025

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
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Performance characteristics of chimerism testing by next generation sequencing.

Matthew F Cusick1, Lauren Clark2, Thuydung Tu2

  • 1Department of Pathology, University of Michigan Medicine, 2800 Plymouth Rd., Building 36, Ann Arbor, MI 48109, USA.

Human Immunology
|November 3, 2021
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers a sensitive and reliable method for chimerism testing, accurately quantifying donor/recipient mixtures down to 1% with high accuracy. This advanced technique provides a valuable alternative to traditional capillary electrophoresis (CE) for clinical samples.

Keywords:
ChimerismHLANGSSNPSTR

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Area of Science:

  • Genetics
  • Molecular Biology
  • Forensic Science

Background:

  • Chimerism testing analyzes biological sample mixtures, traditionally using short tandem repeat (STR) loci with capillary electrophoresis (CE).
  • Next-generation sequencing (NGS) technology enables high-throughput analysis of sequence reads for more accessible mixture quantification.

Purpose of the Study:

  • To evaluate a FORENSIC NGS platform for accurate chimerism percentage measurement.
  • To compare NGS chimerism detection with traditional CE methods.
  • To explore NGS utility in identifying solid organ transplant donors.

Main Methods:

  • Utilized a FORENSIC NGS chimerism platform analyzing both STR and single nucleotide polymorphism (SNP) loci.
  • Compared NGS results with established CE methods for chimerism quantification.
  • Assessed accuracy through six proficiency testing surveys.

Main Results:

  • Achieved accurate chimerism detection down to a 1% limit threshold using NGS.
  • Demonstrated significant correlation between NGS and CE methods from 1% to 10% donor detection.
  • Attained 100% accuracy in proficiency testing over six surveys.
  • Successfully applied NGS for donor identification in solid organ transplantation using SNP profiles.

Conclusions:

  • NGS provides a sensitive, reliable, and accurate alternative to CE for chimerism testing in clinical settings.
  • The NGS platform offers expanded utility for donor selection in solid organ transplantation.
  • NGS facilitates precise quantification of donor-recipient mixtures.