Construction of two plasmids, each containing three different human globin gene fragments

Two plasmids (labelled pUC-2 gamma-beta and pUC-2 beta-delta), each containing three different human globin gene fragments (gamma IVS-II, inter-gamma, and beta IVS-II in pUC-2 gamma-beta, and 5'-beta, 3'-beta, and delta IVS-II in pUC-2 beta-delta) have been constructed. The three gene fragments could be isolated simultaneously from Eco RI/Bam HI double digests of each of the two plasmids using electrophoresis in an 1.0% agarose gel. This facilitates the large-scale isolation of the gene fragments for use as probes in studies such as the analysis of beta-globin gene cluster polymorphisms or of the molecular basis of some hemoglobinopathies.