Digital Microfluidic Thermal Control Chip-Based Multichannel Immunosensor for Noninvasively Detecting Acute...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Cardiovascular Diagnostics

Background:

• Acute myocardial infarction (AMI) diagnosis relies on biomarkers like Myo, CK-MB, and cTnI.
• Current immunoassay methods are time-consuming, complex, and require specialized personnel.
• Limitations in automation hinder rapid point-of-care AMI detection.

Purpose of the Study:

• To develop an automated, rapid, and sensitive detection system for AMI biomarkers.
• To integrate a digital microfluidic (DMF) platform with a thermal control chip for enhanced immunoassay performance.
• To reduce sample and reagent volumes for efficient biomarker analysis.

Main Methods:

• A multichannel digital microfluidic (DMF) thermal control chip was designed and fabricated.
• A sandwich-based immunoassay strategy was employed for biomarker detection.
• Simultaneous detection of three key AMI biomarkers (Myo, CK-MB, cTnI) in human serum was performed.
• A miniaturized temperature control module was integrated for optimal immunoassay conditions.

Main Results:

• The DMF chip enabled automated and multichannel detection of AMI biomarkers.
• Detection of three biomarkers was achieved simultaneously within 30 minutes.
• Reduced sample and reagent consumption (microliters) was demonstrated.
• High sensitivity and diagnostic accuracy for AMI were achieved.

Conclusions:

• The proposed DMF thermal control chip offers a rapid and automated solution for AMI biomarker detection.
• This technology can be deployed in community hospitals for faster diagnosis of acute conditions.
• The system's miniaturization and automation improve diagnostic efficiency and accessibility.