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Does Microfluidic Sperm Sorting Affect Embryo Euploidy Rates in Couples with High Sperm DNA Fragmentation?
Müge Keskin1, Emre Göksan Pabuçcu2, Tufan Arslanca2
1Department of Obstetrics and Gynecology, Faculty of Medicine, Ufuk University, Mevlana Bulvarı No 86-88, Konya Yolu, Balgat, Ankara, 06520, Turkey. mugekeskin1@hotmail.com.
Reproductive Sciences (Thousand Oaks, Calif.)
|November 3, 2021
Summary
Microfluidic sperm sorting (MSS) yields more top-quality blastocysts in couples with repeated implantation failure and high sperm DNA fragmentation (SDF). However, this method did not improve embryo euploidy or live birth rates compared to conventional techniques.
Area of Science:
- Reproductive Medicine
- Genetics
- Infertility Research
Background:
- Male infertility is a significant factor in 30-50% of cases.
- Conventional sperm preparation methods raise concerns about sperm recovery rates.
- Microfluidic sperm sorting (MSS) offers improved selection of motile sperm with reduced sperm DNA fragmentation (SDF).
Purpose of the Study:
- To evaluate if microfluidic sperm sorting (MSS) improves embryo quality and euploidy rates in patients with repeated implantation failure (RIF) and high SDF undergoing preimplantation genetic testing for aneuploidies (PGT-A).
Main Methods:
- A retrospective study comparing MSS with density-gradient centrifugation (DGC) in couples undergoing PGT-A after previous assisted reproductive technology (ART) failures and high SDF.
- Patients were assigned to either the MSS or DGC group for their new cycles.
- Outcomes analyzed included fertilization, euploidy, miscarriage, live birth rates, and blastocyst quality and quantity.
Main Results:
- No significant differences were observed in fertilization rates, euploidy rates, clinical miscarriage, or live birth rates between the MSS and DGC groups.
- The MSS group produced a significantly higher total number of blastocysts and top-quality blastocysts compared to the DGC group.
Conclusions:
- Microfluidic sperm sorting (MSS) increases the yield of top-quality blastocysts but does not enhance embryo euploidy or live birth rates in RIF patients with high SDF.
- Further research is needed to investigate confounding factors affecting embryonic genomic status in the context of high SDF.
