Related Experiment Video
Updated: Jan 20, 2026

Sulforhodamine B Assay: A Sensitive Assay to Measure Drug Resistance Via Determination of Cellular Protein Content in Cancer Cells
Published on: April 30, 2023
Effects of busulfan on the DNA of HL-60 cells as a drug sensitivity assay
Abstract:
We evaluated the formation of DNA-DNA cross links by busulfan as an assay for determining the sensitivity of cells to the drug. HL-60 cells were incubated with busulfan and the effects of the drug on cell growth were compared with the effects of the drug on the alkaline elution pattern of the DNA. While incubation with 100 micrograms/ml for 1 h inhibited cell growth by 50%, drug concentrations of up to 500 micrograms/ml had no effect on the elution pattern of the DNA.
Related Concept Videos
03:57Sulforhodamine B Assay: A Sensitive Assay to Measure Drug Resistance Via Determination of Cellular Protein Content in Cancer Cells
09:41An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
08:25Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
09:19Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
09:39Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
15:41Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

