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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: Oct 14, 2025

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Published on: October 7, 2020

Dissecting mammalian spermatogenesis using spatial transcriptomics.

Haiqi Chen¹, Evan Murray¹, Anubhav Sinha²

¹The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

|November 3, 2021

Summary

This summary is machine-generated.

Spatial transcriptomics reveals gene expression dynamics within seminiferous tubules, uncovering differences between mouse and human testes and how diabetes disrupts male fertility by altering testicular cell organization.

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Last Updated: Oct 14, 2025

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

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Published on: October 7, 2020

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Area of Science:

Reproductive Biology
Genomics
Spatial Transcriptomics

Background:

Single-cell RNA sequencing (scRNA-seq) has identified molecular diversity in spermatogenesis.
However, scRNA-seq cannot capture gene expression dynamics within the native seminiferous tubule microenvironment.

Purpose of the Study:

To create a spatial gene expression atlas of the mammalian testis.
To investigate the spatial organization of testicular cell types and gene programs.
To compare spatial organization between mouse and human testes and in a diabetes infertility model.

Main Methods:

Utilized Slide-seq, a spatial transcriptomics technology, for near-single-cell resolution.
Developed a computational framework to localize testicular cell types within seminiferous tubules.
Combined Slide-seq with in situ RNA sequencing for detailed analysis.

Main Results:

Generated a high-resolution spatial gene expression atlas of mouse and human testes.
Identified spatially patterned genes and gene programs within seminiferous tubules.
Revealed distinct spermatogonial microenvironment cellular compositions in mouse versus human testes.
Demonstrated disrupted spatial cellular organization in seminiferous tubules of diabetic mice.

Conclusions:

Spatial transcriptomics provides critical insights into the dynamics of spermatogenesis within the native testicular context.
Significant interspecies differences exist in testicular cellular organization.
Disruption of spatial organization in seminiferous tubules is a potential mechanism for diabetes-induced male infertility.