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Amplified ribosomal spacer sequence: structure and evolutionary origin.

R Kominami, M Muramatsu

    Journal of Molecular Biology
    |January 5, 1987
    PubMed
    Summary

    Researchers discovered novel amplified spacer sequences in the BALB/c mouse genome. These sequences, unique to BALB/c mice, show surprising homology to Japanese wild-type mouse DNA, suggesting a potential origin.

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    Genomics·1998

    Area of Science:

    • Genomics
    • Molecular Biology
    • Evolutionary Biology

    Background:

    • Ribosomal DNA (rDNA) plays a crucial role in cellular function.
    • Spacer sequences within rDNA are typically non-coding and less studied.
    • Previous research identified pseudogenes and orphons in genomic regions.

    Purpose of the Study:

    • To characterize a newly discovered class of repeated sequences in the mouse genome.
    • To investigate the origin and evolutionary implications of these novel sequences.
    • To compare the newly found sequences with known genomic elements.

    Main Methods:

    • Comparative genomic analysis of mouse ribosomal DNA.
    • Sequence homology studies between different mouse strains and subspecies.
    • Identification and characterization of novel repeated sequence structures.

    Main Results:

    • Discovery of a novel class of repeated sequences composed of tandem arrays of ribosomal spacer sequences.
    • These amplified spacer sequences exhibit a pseudogene-like structure but are distinct from known pseudogenes and orphons.
    • The amplified spacer sequence was exclusively found in the BALB/c mouse genome, absent in ten other laboratory strains and wild-type stocks.
    • A portion of the amplified spacer sequence showed higher homology to the ribosomal DNA of Mus musculus molossinus (Japanese wild-type mouse) than to BALB/c mouse rDNA.

    Conclusions:

    • The amplified spacer sequences represent a unique genomic feature of the BALB/c mouse.
    • The observed homology suggests a possible origin of these sequences from Mus musculus molossinus or a related subspecies.
    • These findings provide insights into mouse genome evolution and the dynamics of repetitive DNA elements.

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