Related Experiment Video
Updated: Oct 14, 2025

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
Published on: March 14, 2014
Itraconazole Inhibits Intracellular Cholesterol Trafficking and Decreases Phosphatidylserine Level in Cervical Cancer
Roze Isono1, Hiroshi Tsubamoto2, Tomoko Ueda1
1Department of Obstetrics and Gynecology, Hyogo College of Medicine. Nishinomiya, Japan.
Background/Aim:
Itraconazole shows anticancer activity in various types of cancer but its underlying mechanism is unclear. We investigated the effect of itraconazole on membrane-associated lipids.
Materials And Methods:
To investigate the influences of itraconazole on cholesterol trafficking, cervical cancer CaSki cells were cultured with itraconazole and analyzed by Filipin staining followed by confocal microscopy. Effect on the glycerophospholipid profiles was analyzed by liquid chromatography/mass spectrometry (LC/MS).
Results:
After itraconazole treatment, Filipin staining revealed cholesterol accumulation in the intracellular compartments, which was similar to the distribution after treatment of U18666A (cholesterol transport inhibitor). LC/MS analysis showed a significant decrease in phosphatidylserine levels and an increase in lysophosphatidylcholine levels in CaSki cells.
Conclusion:
Itraconazole inhibited cholesterol trafficking and altered the phospholipid composition. Alterations in the cell membrane can potentiate the anticancer activity of itraconazole.
More Related Videos
Related Concept Videos
Inhibition of Cdk Activity
Chemotherapy-Induced Nausea and Vomiting: 5-HT3 Receptor Antagonists
Drugs that Stabilize Microtubules
Drugs that Destabilize Microtubules
Tumor Immunotherapy

