Jove
Visualize
Contact Us

Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

4.7K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.7K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

355
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
355

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnetite-enhanced humic substances-driven anaerobic oxidation of methane: Insights into extracellular electron transfer mechanisms and microbial interactions.

Bioresource technology·2026
Same author

Low-Dose Buprenorphine Initiations During Opioid and Sedative Weaning in Mechanically Ventilated Neurocritical Care Patients: A Retrospective Pilot Cohort Study.

Neurocritical care·2026
Same author

Egyptian rousette bat humoral immunity to H9 influenza hemagglutinin.

bioRxiv : the preprint server for biology·2026
Same author

CEPI Workshop Report: Applying Disease X Vaccine Library and Knowledge Base Approaches to Severe Fever with Thrombocytopenia Syndrome (SFTS).

Vaccines·2026
Same author

Lactic alkalosis in a patient on veno-venous extracorporeal membrane oxygenation: A case report.

The International journal of artificial organs·2026
Same author

A Narrative Summary of Strategies to Mitigate Intradialytic Hypotension in Poisoned Patients Requiring Extracorporeal Toxin Elimination.

Journal of medical toxicology : official journal of the American College of Medical Toxicology·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 14, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

2.1K

Selection for constrained peptides that bind to a single target protein.

Andrew M King1,2, Daniel A Anderson1, Emerson Glassey1

  • 1Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Nature Communications
|November 4, 2021
PubMed
Summary

We developed a method to engineer custom peptides in E. coli for targeted protein binding. This approach successfully identified a peptide that binds to the SARS-CoV-2 Spike protein, paving the way for new therapeutics.

More Related Videos

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.2K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.2K

Related Experiment Videos

Last Updated: Oct 14, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

2.1K
Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.2K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Peptide secondary metabolites possess diverse pharmacological functions, including antibiotic activity and signaling.
  • Developing methods to engineer peptides for specific protein targets is crucial for therapeutic and diagnostic applications.

Purpose of the Study:

  • To present a novel method for designing and screening large libraries of modified peptides in Escherichia coli.
  • To identify peptides that bind to a specific protein target of interest using an in vivo screening system.

Main Methods:

  • Utilized modified enzymes from microbial RiPP (ribosomally synthesized and post-translationally modified peptide) pathways to produce constrained peptide scaffolds.
  • Diversified peptide scaffolds to create large libraries for screening.
  • Employed an intein-based system where target binding releases an RNA polymerase sigma factor, driving selectable marker expression.

Main Results:

  • Successfully screened a library for binding to the SARS-CoV-2 Spike receptor binding domain.
  • Identified a 1625 Da constrained peptide (AMK-1057) with a binding affinity of 990 ± 5 nM.
  • The identified peptide demonstrated comparable binding affinity to an ACE2-derived peptide.

Conclusions:

  • Demonstrated a generalizable method for identifying constrained peptides that bind to specific protein targets.
  • This approach serves as a foundational step towards developing "molecular glues" for therapeutic and diagnostic applications.
  • The engineered peptides show potential for future drug discovery and development.