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Integrative Characterization of the Role of IL27 In Melanoma Using Bioinformatics Analysis
Chunyu Dong1, Dan Dang2, Xuesong Zhao1
1Department of Pediatric Surgery, The First Hospital of Jilin University, Changchun, China.
Frontiers in Immunology
|November 4, 2021
Summary
Interleukin-27 (IL27) enhances anti-tumor immunity and improves prognosis in melanoma. High IL27 expression correlates with better survival and response to immunotherapy, suggesting its potential in cytokine-based treatments.
Area of Science:
- Oncology
- Immunology
- Genetics
Background:
- Interleukin-27 (IL27) exhibits dual roles in cancer, but its impact on melanoma's tumor microenvironment (TME), immunotherapy response, and patient prognosis is not well understood.
- This study investigates the multifaceted effects of IL27 in melanoma patients.
Purpose of the Study:
- To elucidate the role of IL27 in the melanoma tumor microenvironment (TME).
- To assess the association of IL27 with immunotherapy response and patient prognosis in melanoma.
- To explore IL27's potential as a therapeutic target in melanoma.
Main Methods:
- Utilized RNA-seq, drug sensitivity, and clinical data from TCGA, GEO, CCLE, and CTRP.
- Performed survival analyses (log-rank, Cox regression) and functional enrichment analyses (DAVID, GSEA).
- Assessed immune infiltrates using ssGSEA and classified tumors (hot/cold) with ConsensusClusterPlus.
Main Results:
- High IL27 expression correlated with improved survival and was an independent prognostic predictor.
- IL27 positively associated with radiotherapy response and negatively with Breslow depth.
- Elevated IL27 promoted immune response, pyroptosis, autophagy, and apoptosis, correlating with cytotoxic cytokines and enhanced CD8/Treg ratio, potentially via suppressing β-catenin signaling.
Conclusions:
- IL27 is linked to enriched CD8+ T cells, favorable therapeutic outcomes, and better prognosis in melanoma.
- IL27 shows potential as a modulator for cytokine-based immunotherapy in melanoma treatment.
