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Nxhl Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin.
Honglin Luo1,2, Yongde Zhang1, Yanfei Deng3
1Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.
Frontiers in Cell and Developmental Biology
|November 4, 2021
Summary
Researchers discovered a new gene, nxhl (New XingHuo light), crucial for vascular integrity and angiogenesis. This finding offers a potential therapeutic target for diseases involving blood vessel formation, including cancer.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- Precise regulation of angiogenesis is vital for organ development, wound repair, and tumor progression.
- Vascular integrity and angiogenesis are complex processes involving numerous genetic factors.
Purpose of the Study:
- To identify and characterize a novel gene involved in angiogenesis and vascular integrity.
- To elucidate the molecular mechanism by which the novel gene regulates angiogenesis.
Main Methods:
- Bioinformatic analysis of gene co-expression.
- Zebrafish knockdown studies to observe vascular defects.
- In vitro studies on human endothelial cells.
- Investigation of protein interactions (nxhl, NCL, VE-PTP).
Main Results:
- A novel conserved gene, nxhl (New XingHuo light), was identified with essential roles in development and vascular integrity.
- nxhl knockdown in zebrafish led to edema, circulatory defects, and abnormal vascularization.
- nxhl was found to interact with nucleolin (NCL) and target VE-PTP, regulating angiogenesis via a novel nxhl-NCL-VE-PTP axis.
- nxhl deficiency attenuated tumor invasion and key proteins involved in angiogenesis and epithelial-mesenchymal transition (EMT).
Conclusions:
- nxhl plays a critical role in regulating angiogenesis and vascular integrity.
- The nxhl-NCL-VE-PTP axis represents a newly identified pathway controlling vascular formation.
- nxhl is a potential therapeutic target for modulating angiogenesis, particularly in cancer treatment.
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