Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

357
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
357
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

5.1K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
5.1K
Antibiotic Selection00:57

Antibiotic Selection

56.6K
Overview
56.6K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

135
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
135
Antimicrobial Proteins01:23

Antimicrobial Proteins

9.5K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-reactive anti-prophage antibodies and bacterial heteroresistance implicated in phage therapeutic failure.

Nature medicine·2026
Same author

Enhanced Spread of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> in ICU Environment During a COVID-19 Upsurge Period in China.

Infection and drug resistance·2026
Same author

Challenges and Strategies in Managing β-Lactam-Resistant Pseudomonas aeruginosa in China.

The Journal of infectious diseases·2026
Same author

Molecular Epidemiology and Persistence of Multidrug-Resistant Acinetobacter baumannii in Zhejiang, China (2015-2020): Insights Into Resistance, Virulence, and Transmission Dynamics.

The Journal of infectious diseases·2026
Same author

Evaluation of the performance of a novel chemiluminescence immunoassay for cryptococcal antigen detection.

Microbiology spectrum·2026
Same author

Heterogeneous multicopy of bla<sub>CTX-M</sub> variants on the same plasmid enhances evolutionary adaptability in clinical Klebsiella pneumoniae.

Nature communications·2026

Related Experiment Video

Updated: Oct 14, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.8K

Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope

Xiaoqing Wang1,2, Belinda Loh3, Fernando Gordillo Altamirano4,5

  • 1Zhejiang University-University of Edinburgh (ZJU-UoE) Institute, Zhejiang University, Haining, People's Republic of China.

Emerging Microbes & Infections
|November 5, 2021
PubMed
Summary

Phage resistance in Acinetobacter baumannii involves changes to the bacterial envelope, reducing virulence. Interestingly, these phage-resistant bacteria show increased sensitivity to colistin, offering a potential therapeutic strategy.

Keywords:
Phageantibiotic resensitationcolistin resistancephage adsorptionphage therapyphage-resistancevirulence

More Related Videos

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

26.7K
Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux&#45;Deficient Bacterial Strain and a Single&#45;Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

783

Related Experiment Videos

Last Updated: Oct 14, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.8K
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

26.7K
Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux&#45;Deficient Bacterial Strain and a Single&#45;Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

783

Area of Science:

  • Microbiology
  • Bacteriology
  • Genetics

Background:

  • Multidrug-resistant bacterial infections pose a significant global health threat.
  • Phage therapy is a promising alternative to antibiotics, but bacterial resistance to phages is a challenge.
  • Acinetobacter baumannii is a critical priority pathogen with limited treatment options.

Purpose of the Study:

  • To elucidate the mechanisms of phage resistance in Acinetobacter baumannii.
  • To characterize a novel phage, Phab24, and its interaction with A. baumannii.
  • To assess the impact of phage resistance on bacterial virulence and antibiotic sensitivity.

Main Methods:

  • Isolation and characterization of a novel phage (Phab24) targeting Acinetobacter baumannii.
  • Generation and whole-genome sequencing of phage-resistant A. baumannii mutants.
  • Phenotypic characterization including virulence assays in Galleria mellonella and antibiotic sensitivity testing.

Main Results:

  • Phage-resistant A. baumannii strains exhibited mutations affecting the bacterial capsule and outer membrane.
  • Phage Phab24 utilizes the bacterial capsule as its primary receptor.
  • Phage-resistant isolates displayed reduced virulence and, notably, increased sensitivity to colistin.

Conclusions:

  • Phage resistance in A. baumannii is mediated by alterations in the bacterial envelope structure.
  • The emergence of phage resistance can unexpectedly re-sensitize bacteria to antibiotics like colistin.
  • This phage-induced antibiotic hypersensitivity presents a potential strategy for combating multidrug-resistant infections.