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Related Experiment Video

Updated: Oct 14, 2025

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Spectroscopic ellipsometry methods for brevetoxin detection.

Mustafa Oguzhan Caglayan1, Zafer Üstündağ2, Samet Şahin1

  • 1Department of Bioengineering, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey.

Talanta
|November 5, 2021
PubMed
Summary

Spectroscopic ellipsometry (SE) and attenuated internal reflection spectroscopic ellipsometry (TIRE) biosensors effectively detect brevetoxin B (BTX). While competitive, aptamer selectivity for BTX analogs needs improvement for enhanced biosensing accuracy.

Keywords:
Attenuated total internal reflection ellipsometryBrevetoxinParalytic shellfish poisoning toxinsSeafoodSurface plasmon resonance

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Area of Science:

  • Biosensing
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Label-free biosensing is crucial for real-time monitoring.
  • Surface Plasmon Resonance (SPR) offers high sensitivity and real-time measurement capabilities.
  • Spectroscopic Ellipsometry (SE) and Attenuated Internal Reflection Spectroscopic Ellipsometry (TIRE) are advanced ellipsometry techniques for biosensing.

Purpose of the Study:

  • To develop and compare SE and TIRE-based biosensors for brevetoxin B (BTX) detection.
  • To evaluate the analytical performance and selectivity of existing and newly derived anti-BTX aptamers.
  • To assess the application of these sensors in real-world sample matrices.

Main Methods:

  • Development of SE and TIRE biosensors utilizing pre-existing and novel anti-BTX aptamers.
  • A new aptamer sequence was generated using predictive modeling and exclusion methods.
  • Analytical characterization including detection range, limits, and selectivity against BTX analogs.

Main Results:

  • Both SE and TIRE sensors demonstrated competitive analytical performance for BTX detection.
  • BTX detection ranges were 0.05–1600 nM (TIRE) and 0.5–2000 nM (SE).
  • Previously reported aptamers showed poor selectivity against BTX analogs, and the derived aptamer had even lower selectivity.

Conclusions:

  • SE and TIRE biosensors are viable for sensitive BTX detection in complex samples.
  • The selectivity of anti-BTX aptamers against analogs remains a challenge, particularly at low concentrations.
  • Further optimization of aptamer sequences is necessary to improve sensor specificity for accurate toxin monitoring.