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Published on: August 7, 2017
Correlation between Serum Interleukin-17 level and Serum Reactive Oxygen Species levels among Children experiencing
Marwa Abdelhafeez1, Noura Mostafa Mohamed2,3
1Department of Otorhinolaryngology, Faculty of Medicine, Minia University, Minia, Egypt.
Insights
Interleukin-17 (IL-17) serum levels correlate with increased reactive oxygen species (ROS) in children with otitis media with effusion (OME). This suggests the IL-17 pathway plays a role in OME development by elevating ROS.
Area of Science:
- Pediatrics
- Immunology
- Otolaryngology
Background:
- Otitis media with effusion (OME) is common in children.
- Identifying inflammatory mediators may lead to nonsurgical treatments for OME.
- The role of IL-17 and its effect on oxidative stress in OME requires further investigation.
Purpose of the Study:
- To investigate the relationship between serum interleukin-17 (IL-17) and reactive oxygen species (ROS) in children with OME.
- To determine if IL-17 levels influence ROS and antioxidant balance in OME patients.
Main Methods:
- A case-control study involving 24 children with OME and 24 healthy controls.
- Clinical assessments included otoscopy, audiometry, and tympanometry.
- Serum levels of IL-17 and ROS markers (NO, MDA, MPO, CT, SOD, GPX) were measured.
Main Results:
- Significant differences in serum nitric oxide (NO), myeloperoxidase (MPO), and malondialdehyde (MDA) levels were observed between OME patients and controls (p < 0.05).
- Serum levels of MDA, NO, and MPO showed a positive correlation with IL-17 levels in children with OME.
- No significant differences in superoxide dismutase (SOD) or glutathione peroxidase (GPX) were found between groups.
Conclusions:
- The IL-17 pathway appears to contribute to OME pathogenesis.
- Elevated ROS levels, indicated by increased MDA, NO, and MPO, are associated with IL-17 in OME.
- These findings support a potential role for IL-17 in the inflammatory processes of OME.
Abstract:
Introduction The detection of inflammatory mediators in the serum of children with have otitis media with effusion (OME) and their correspondence with clinical considerations may enable the use of a modern nonsurgical curative treatment for OME. Objective To determine the relation between interleukin-17 (IL-17) serum level and reactive oxygen species (ROS) serum levels in children suffering from OME and to disclose if any variation occurs in the level of IL-17 Will affect the ROS and antioxidant equilibrium in the serum, which indicates the entire body's reaction to OME. Methods The present study was a case-control study. A total of 24 children experienced OME, and 24 healthy controls were recruited. All participants in the study were subjected to a systematic clinical investigation including otoscopic, audiometric, and tympanometric examination. Also, venous blood samples were collected from all children to determine the levels of IL-17 and ROS. Results The mean ± standard deviation (SD) age ranges of the patients and the control group were 6.8 ± 2.7 and 6.2 ± 3.4 years, respectively. A stylistically significant difference in the levels of serum nitric oxide (NO), catalase (CT), myeloperoxidase (MPO), and malondialdehyde (MDA) ( p < 0.05) was detected between OME and control patients. No significant difference was found in serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) between OME and control patients. The serum levels of MDA, NO, and MPO positively correlated with the serum levels of IL-17 in OME patients. Conclusion In the present study, there is a reasonable role of the IL-17 pathway in OME pathogenesis through an increase in ROS levels.
