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Positive interactions are common among culturable bacteria.

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Positive interactions between soil bacteria are common, not rare, and often occur when strains consume different carbon sources. This finding aids in cultivating microbes and designing microbial communities.

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Area of Science:

  • Microbial ecology
  • Synthetic biology
  • Biotechnology

Background:

  • Interspecies interactions are crucial for microbial community structure and function.
  • Positive interactions can enhance microbial diversity and productivity but their prevalence is poorly understood.
  • Understanding these interactions is key for applications in natural and engineered systems.

Purpose of the Study:

  • To investigate the prevalence and conditions of positive interspecies interactions among soil bacteria.
  • To identify the types of interactions and the environmental factors influencing them.
  • To explore the potential of positive interactions for microbial cultivation and community design.

Main Methods:

  • Utilized kChip, an ultrahigh-throughput coculture platform.
  • Assayed 180,408 interactions among 20 soil bacterial strains.
  • Tested interactions across 40 different carbon environments.

Main Results:

  • Positive interactions were found to be common, contrary to previous assumptions.
  • These interactions primarily occurred as parasitisms between strains with differing carbon consumption profiles.
  • Non-growing strains were significantly promoted by strongly growing strains (85% of cases).

Conclusions:

  • Positive microbial interactions are prevalent and predictable based on carbon utilization.
  • This suggests a straightforward approach for microbial cultivation and microbiome engineering.
  • Findings support the design of microbial consortia leveraging positive interactions.